Lnc-RT HeroTM I(With gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA)

Lnc-RT Hero^TM I(With gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA)

Description:

Lnc-RT Hero^TM I (With gDNase) is a reverse transcription system specially developed for lncRNA to quickly remove genomic DNA contamination. 5×gDNase Mix can quickly remove the remaining genome in RNA at 42°C for 2 minutes, effectively avoiding the interference of genome on qPCR results.

5×L-RT HeroTM Mix contains Foregene LncRNA Reverse Transcriptase specially developed by Foregene, which is a new type of reverse transcriptase specifically for lncRNA and other long RNA complex templates, with stronger RNA affinity and higher reversal recording efficiency. The optimized system makes the reverse transcription rate faster and can easily transcribe RNA templates with high GC content and complex secondary structure. The first strand cDNA synthesis can be completed at 42°C in 15 minutes.

Specifications:

25×20μl Rxns, 50×20μl Rxns

Kit components

Kits component information

-5×gDNase Mix: gDNA removing agent, it is necessary to use the reagent in accordance with the operating instructions before the RT reaction is performed (the internal glycerol content may not be frozen, and it may not be frozen, which belongs to normal phenomena).

- 5×L-RT HEROTM MIX: Contains Foregene Lncrna Reverse Transcriptase, RNase Inhibitor, DNTPS, Stabilifer, Enhancer, Optimizer, Oligo (DT) 18 primer).

Features&advantages:

-Efficient ability to remove gDNA, which can remove gDNA in the template within 2 minutes.

-It can efficiently reverse transcription of lncRNA. When the reverse transcription product is subjected to qPCR, the Ct value is lower than that of conventional reverse transcription system.

-Efficient reverse transcription system, it only takes 15 minutes to complete the synthesis of the first strand cDNA.

-Complex templates: templates with high GC content and complex secondary structure can also be reversed with high efficiency.

-High-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.

-The reverse transcription system has high thermal stability, the optimal reaction temperature is 42℃, and it still has good reverse transcription performance at 50℃.

Kit application:

lncRNA relative quantitative analysis.

lncRNA absolute quantitative analysis.

It can quickly and accurately analyze trace RNA such as RNA virus.

Reverse transcription of RNA templates with high GC content or complex secondary structure.

Workflow:

Precautions: (Please read the precautions carefully before using the kit)

- Template recommends using fresh samples or RNA preserved under -80 ° C (RNA should avoid repeated freeze-thaw, ensuring RNA integrity, no degradation).

- In order to avoid RNASE pollution, the experimental operation is carried out in RNase-Free space; the gun head and PCR centrifuge must be guaranteed to be RNASE-Free; and wear disposable gloves and masks.

-Before using, 5 × GDNase Mix and 5 × L-RT HERO^TM MIX are placed on ice make it completely melted, and the light bullet is mixed; the system is formulated, please operate on an ice bath to improve the performance of the kit, improve the performance of the kit PCR amplification specificity.

- 5 × L-RT HERO^TM MIX has added an optimized ratio reverse transcription primer without adding any primers to additional additional primers.

-For the complex RNA template of the secondary structure, the reaction temperature can be increased to 50 ° C

Storage and Shelf life:

The Super Premix PCR Kits should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.