Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | China |
| Brand Name: | FOREGENE |
| Certification: | ISO CE |
| Model Number: | SC-01012 |
| Minimum Order Quantity: | 10000 tests |
|---|---|
| Price: | 2 usd per tests |
| Packaging Details: | Packing with safe trans carton(trans with low temperature ice box) |
| Delivery Time: | 7-15 working days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union |
| Supply Ability: | 1000000 tests per month |
| Product Name: | Covid 19 RT PCR Test Kits | Packing: | 96 Tests Per Box(kits) |
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| Storage: | -20 Degree | Usage: | For SARS-Cov-2 Virus Detection |
| Brand: | FOREGENE | MOQ: | 100 Kits |
| High Light: | 2 genes Covid 19 RT PCR Test Kits,Multiplex SARS-Co V-2 Nucleic Acid Detection Kit,SARS-CoV-2 Nucleic Acid Detection Kit |
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Multiplex PCR Fluorescent Probe Method SARS-CoV-2 Nucleic Acid Detection Kit
Intended Use
This kit uses Real-time RT PCR technology (rRT-PCR) for qualitative detection of SARS-CoV-2 nucleic acids in human nasopharyngeal or oropharyngeal swab samples.
Principle of testing
This primer and probe sets are designed for the detection of the conserved sequences of SARS-CoV-2 (ORF1ab gene and N gene). The kit contains an Internal Control that is used to assess specimen quality.
Real-Time PCR technology utilizes polymerase chain reaction (PCR) for the amplification of specific target sequences and target specific probes for the detection of the amplified RNA. The probes are labelled with fluorescent reporter and quencher dyes.
Quick Details
◮ Specifications: 48 Rxns/kit, 96 Rxns/kit
◮ Specifificity:96.72%
◮ Sensitivity:97.92%
◮ Applicable specimen types
Nasopharyngeal Swab or Oropharyngeal Swab
◮ Applicable instrument
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Components
|
No. |
Component |
Amount |
Main components |
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|
48 Rxns |
96 Rxns |
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|
1 |
Nucleic acid release agent |
1.4mL/tube |
2 tubes |
5.3 mL/bottle |
1 bottle |
Surfactant |
|
2 |
RNA protectant |
27 μL/tube |
1 tube |
53 μL/tube |
1 tube |
RNase inhibitor |
|
3 |
SARS-CoV-2 reaction solution |
800 μL/tube |
1 tube |
1600 μL/tube |
1 tube |
Primer, probe, reaction buffer, dNTP |
|
4 |
SARS-CoV-2 enzyme mixture |
80 μL/tube |
1 tube |
160 μL/tube |
1 tube |
Hot start Taq enzyme, M-MLV enzyme |
|
5 |
SARS-CoV-2 positive control |
100 μL/tube |
1 tube |
100 μL/tube |
1 tube |
Recombinant plasmid containing target fragment, RNA |
|
6 |
SARS-CoV-2 negative control |
1200 μL/tube |
1 tube |
1200 μL/tube |
1 tube |
TE buffer |
Features&advantages
◮ Direct PCR
No need for nucleic acid extaction kit and System, completed 96 specimen in 1 hour or so.
◮ Low equipment requirement and highly flflexible use
Only need Real-Time PCR system.
◮ LoD and high sensitivity
Detection sensitivity can reach the lowest level as 500 copies/ml.
How to use?
Method 1: Direct PCR method
Method 2: Viral RNA Isolation + PCR method
Detection procedure
Cold Chain Transportation and Storage
Sealed from light and stored at -20±5℃;
Shelf life:
1 year
Ordering Information:
Contact Person: Foregene
Tel: +8613880676215
