HELLO, friends!
After doing nucleic acid extraction for so many years, everyone is well aware of the fact that the quality of nucleic acid extraction is the key to the success and accuracy of subsequent experiments.
Many times, we just care whether the ratio of OD260/OD280 is within the optimal range. Just like this...
As the saying goes, you can't speak too much, and you can't be happy too early.
First listen to Editor tell you a little story!
Long, long ago, protein and RNA were close friends.
Mr Protein is more shy.
So it has always been the active and cheerful RNA to play with it.
One day.
RNA told Mr Protein that it was going to be adopted by others, and Mr Protein was very sad.
RNA is also very reluctant to Mr protein, and said: "How about I sneak you away, you hide in my clothes, try not to be seen by others, okay?"
Mr. Protein broke into a smile and happily agreed.
On the day of adoption, Mr Protein really hid in RNA's clothes and was not found. He followed the adopted person back home and lived a happy life ever since.
Such beautiful fairy tales often happen in laboratories that do nucleic acid extraction.
You thought you only took RNA, Sorry!
In fact, it's just that you don't know that Mr Protein is secretly hiding.
Do you think that you want to make Mr Protein appear with only one OD260/OD280?
Too young! Too simple!
Let us use the most sensitive silver staining method to help you see the proteins hidden in RNA!
Product used | OD260/280 | Measuring instrument |
Foregene | 2.09 |
Thermo ScientificTM NanoDropTM One Ultra-trace UV-Vis Spectrophotometer |
Trizol | 1.91 |
Comparison of RNA extraction with Foregene Cell Total RNA Extraction Kit
and T Company's Trizol Reagent
We can clearly see the banding on lane 4 (Trizol), while lane 3 (Foregene) is relatively clean. The results confirmed that the RNA protein impurities extracted by the Trizol method were still many drops! And lane 3 (Foregene) has almost no protein impurities because the experimenter used the Foregene RNA extraction kit! (The advertisement came so unexpectedly)
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