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Fast Room Temperature Operation Stool DNA Isolation Kits For Genomic Dna Extraction

Fast Room Temperature Operation Stool DNA Isolation Kits For Genomic Dna Extraction

  • Fast Room Temperature Operation Stool DNA Isolation Kits For Genomic Dna Extraction
  • Fast Room Temperature Operation Stool DNA Isolation Kits For Genomic Dna Extraction
  • Fast Room Temperature Operation Stool DNA Isolation Kits For Genomic Dna Extraction
Fast Room Temperature Operation Stool DNA Isolation Kits For Genomic Dna Extraction
Product Details:
Place of Origin: Sichuan, China
Brand Name: Foregene
Certification: CE, ISO
Model Number: 5T, 25T, 50T, 100T, 250T
Payment & Shipping Terms:
Minimum Order Quantity: 1 kit
Price: Negotiable
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 100000kits per month
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Detailed Product Description
Factory: Foregene Product Name: Nucleic Acid Purification DNA Isolation Kits Stool DNA Isolation Kit Without RNase Contamination
CAT NO.: DE-05711, DE-05712, DE-05713, DE-05714, DE-05715 Type: DNA-Only Column
Application: Purification Of Genomic DNA Operation Temperature: Room Temperature
High Light:

Operation Stool DNA Isolation Kits

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Genomic DNA Isolation Kits 250T

Fast room temperature operation Stool DNA Isolation kits Extraction kits for genomic dna extraction

 

Description

 

This kit provides a quick and easy method to extract genomic DNA from bacteria and food residues from stool samples from various sources. There are a large number of inhibitors in stool samples. Even if these substances are present in a small amount in the purified DNA, they will affect downstream reactions, such as PCR and restriction enzyme digestion. Therefore, the key to purification of fecal DNA is how to effectively remove inhibitors in feces.

This kit uses DNA-Only Column that can specifically bind DNA, a brand-new Foregene Protease and a unique buffer system, which can effectively remove various inhibitors in feces, without organic solvent extraction or ethanol and isopropanol precipitation. The DNA extraction operation from stool samples can be completed in 40 minutes.

 

Fast Room Temperature Operation Stool DNA Isolation Kits For Genomic Dna Extraction 0

 

Kit conponents

 

Stool DNA Isolation Kit

Kit contents DE-05711 DE-05712 DE-05713 DE-05714 DE-05715
5 T 25 T 50 T 100T 250T
Buffer SL1 3.5ml 18ml 35ml 70ml 175ml
Buffer SL2 3.5ml 18ml 35ml 70ml 175ml
Buffer SL3 3.5ml 18ml 35ml 70ml 175ml
Buffer PW 3ml 15ml 30ml 60ml 150ml
Buffer WB 2.5ml 12.5ml 25ml 50ml 125ml
Buffer EB 1.5ml 6ml 15ml 25ml 75ml
Buffer TE 6ml 30ml 60ml 120ml 300ml
Foregene Protease 280μl 1.4ml 1.4mlx2 5.6ml 14ml
Lysozyme 50mg 250mg 500mg 500mgx2 500mgx5
DNA-Only Column 5 sets 25 sets 50 sets 100 sets 250 sets
IFU 1 piece 1 piece 1 piece 1 piece 1 piece

 

Features&advantages

 

- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA from the genomic DNA without adding RNase during the experiment, preventing the laboratory from being contaminated by exogenous RNase.

- Fast speed: The operation is simple, and the fecal genomic DNA extraction operation can be completed within 40 minutes.

- Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.

- Safety: No organic reagent extraction is required.

- High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

 

Product INFO

 

Model Spin-column type Purification components Foregene Spin column, reagent
Flux 1-24 samples Preparation time

40min

(24 samples)

Centrifuge Table centrifuge Tissue enzymatic hydrolysate separation centrifugal separation
Purification column DNA load carrying capacity 80μg Liquid volume of spin column 800μl
Elution volume 60-200μl Stool sample processing volume 50-200mg

 

 

Genomic DNA Application

 

The genomic DNA purified by Stool DNA Isolation Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern hybridization, library construction and other experiments.

 

Quality Control

 

According to FOREGEN’s Total Quality Management System, each batch of Stool DNA Isolation kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

 

Kit components

◆ Lysozyme: Enzymatically hydrolyze the cell wall of positive bacteria.

◆ Buffer TE: Used to prepare 100mg/ml Lysozyme solution and provide lysozyme enzymatic digestion environment.

◆ Buffer SL1: Provide sample protease enzymolysis environment.

◆ Foregene Protease: Enzymatically digest the sample in a protease enzymatic environment to release genomic DNA.

◆ Buffer SL2: to remove grease impurities.

◆ Buffer SL3: Inactivate protease and provide a DNA loading environment.

◆ Buffer PW: Remove impurities such as protein and RNA in DNA.

◆ Buffer WB: Remove residual salt ions in DNA.

◆ Buffer EB: elute the DNA on the purification column membrane.

◆ DNA-only Column: Specific adsorption of genomic DNA in the lysate.

 

DNA-Only Column Characters

Maximum binding capacity 80μg
Maximum loading volume 800μl
Longset DNA fragment 23kb
Minimum elution volume* 60μl
Selection of samples Newly taken stool samples
Maximum amount of starting material* 200mg stool sample

*: The minimum elution system of 60μl is a reasonable recommended volume considering DNA recovery and concentration. If you want to increase the yield of DNA, you can appropriately increase the volume of the eluate; if you want to increase the concentration of purified DNA, at the expense of part of the DNA yield, reduce the volume of the eluate appropriately, such as using a 40μl elution system, In order to get a higher concentration of DNA.

 

Genomic DNA extraction yield and purity

 

The amount of fecal genomic DNA extracted is related to factors such as the source, storage time, and moisture content of the fecal sample. Stool DNA Isolation Kit is used to process stool samples from various sources, and the amount and purity of genomic DNA obtained are shown in the following table:

Sample source Sample dosage(mg) DNA yield(μg) OD260/280 OD260/230
cattle 200 4-6 1.7-1.9 1.7-1.9
Domestic pig 200 4-6 1.7-1.9 1.8-1.9
Poultry (chicken) 200 4-6 1.7-1.9 1.7-1.8

Note: The data in this table is for reference only. In actual operation, due to the storage conditions of the materials used, operating proficiency and other factors, the data obtained may differ slightly from the data in this table.

 

 

Genomic DNA fragment size

Stool DNA Isolation Kit uses silica gel membrane columns to separate and purify fecal genomic DNA. If the sample is stored well or the sample is fresh, the purified genomic DNA fragment size is around 23kb; if the sample is stored for a long time or the operation is improper during the extraction process, It will cause the genomic DNA to degrade into diffuse bands. As shown in the figure:

Fast Room Temperature Operation Stool DNA Isolation Kits For Genomic Dna Extraction 1

 

Storage and Shelf life

 

- This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

- Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months), its activity and stability will be better when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.

- Dry Lysozyme powder is stored at -20°C, the prepared Lysozyme solution is divided into small portions and stored at -20°C.

Contact Details
Foregene Co., Ltd.

Contact Person: Maggie

Tel: +8615281067355

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