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Foregene Co., Ltd.
Foregene Co., Ltd.

Foregene Co., Ltd.

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Laboratory Bacterial DNA Isolation Kit Bacterial DNA Extraction Purification Kits

Laboratory Bacterial DNA Isolation Kit Bacterial DNA Extraction Purification Kits

  • Laboratory Bacterial DNA Isolation Kit Bacterial DNA Extraction Purification Kits
  • Laboratory Bacterial DNA Isolation Kit Bacterial DNA Extraction Purification Kits
  • Laboratory Bacterial DNA Isolation Kit Bacterial DNA Extraction Purification Kits
Laboratory Bacterial DNA Isolation Kit Bacterial DNA Extraction Purification Kits
Product Details:
Place of Origin: Sichuan, China
Brand Name: Foregene
Certification: CE, ISO
Model Number: 50T, 100T, 250T
Payment & Shipping Terms:
Minimum Order Quantity: 1 kit
Price: Negotiable
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 100000kits per month
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Detailed Product Description
Factory: Foregene Product Name: Bacterial DNA Isolation Kit Laboratory Use Extraction And Purification Kits For Bacterial DNA
CAT NO.: DE-05311, DE-05312, DE-05313 Type: DNA-Only Column
Application: Purify Genomic DNA Operation Temperature: Room Temperature
High Light:

Laboratory Bacterial DNA Isolation Kit

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50T Bacterial DNA Isolation Kit

Bacterial DNA Isolation Kit laboratory use extraction and purification kits for bacterial DNA

 

 

Description

 

Bacterial DNA Isolation kit provides a quick and easy method of extracting genomic DNA from cultures of bacteria (Gram-negative and Gram-positive) from various sources; it can process less than 3ml of bacterial cultures in the logarithmic growth phase (1×109 bacteria). The kit is matched with high-efficiency Foregene Protease, which allows 15-50μg of high-quality genomic DNA to be extracted within 1 hour. In addition, the kit can also extract genetic material other than the genome, such as plasmids, Cosmid, BAC, etc.

The DNA-only silica gel matrix material used in the spin column is a unique new material of the company, which can efficiently and specifically adsorb DNA. The maximum adsorption capacity for DNA is 80μg. The unique buffer and elution system can maximize the removal of RNA, Impurity proteins, ions and other organic compounds in cells. The extracted genomic DNA fragment is large, high purity, stable and reliable quality, and the size of the DNA fragment is stable at about 23kb.

 

Kit conponents

 

Bacterial DNA Isolation Kit
Kit contents DE-05311 DE-05312 DE-05313
50 T 100 T 250 T
Buffer ML1 25ml 50ml 125ml
Buffer ML2 25ml 50ml 125ml
Buffer PW 30ml 60ml 150ml
Buffer WB 25ml 50ml 125ml
Buffer EB 10ml 20ml 50ml
Foregene Protease 1.25ml 1.25ml x2 6.25ml
Lysozyme 250mg 500mg 500mg x2
Buffer TE 5ml 8ml 10ml
DNA-Only Column 50 sets 100 sets 250 sets
IFU 1 piece 1 piece 1 piece

 

Features&advantages

- No RNase contamination: No need to add RNase to remove the RNA in the genomic DNA, avoiding the laboratory from being contaminated by exogenous RNase.

- Fast speed: Foregene Protease has higher activity than similar proteases and can digest broken bacterial samples in a short time.

- Convenience: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.

- Safety: no organic reagent extraction is required.

- High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

 

Genomic DNA Application

 

The genomic DNA purified by the Bacterial DNA isolation kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern hybridization, library construction and other experiments.

 

Genomic DNA extraction yield and purity

 

The amount of genomic DNA obtained by using the Bacterial DNA isolation kit to process bacteria is related to the source of the sample, storage time, culture time and other factors. Generally, the bacterial genomic DNA obtained under normal circumstances is the sum of the DNA in the bacterial cell, and the genomic DNA yield of the bacteria cultured overnight in 3ml is about 15-50μg. In actual operation, the amount obtained may differ slightly from the data.

 

Genomic DNA fragment size

 

The bacterial genomic DNA extraction kit uses a silica membrane column to separate and purify bacterial genomic DNA. The purified genomic DNA fragments are all around 23kb in size. As shown in the figure:

Laboratory Bacterial DNA Isolation Kit Bacterial DNA Extraction Purification Kits 0

 

Storage and Shelf life

 

- This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to put the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

- Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months); when stored at 4°C, its activity and stability will be better, so it is recommended to store it at 4°C, remember not to keep it at -20°C.

- Lysozyme is a dry powder, which is active when stored at 2-8°C for a long time (3 months). For longer storage, please store it at -20°C.

 

Laboratory Bacterial DNA Isolation Kit Bacterial DNA Extraction Purification Kits 1

Contact Details
Foregene Co., Ltd.

Contact Person: Maggie

Tel: +8615281067355

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Foregene Co., Ltd.
Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
Tel:86-028-86050301
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