Plant Total RNA Extraction Kit For Low Polysaccharide And Polyphenol Samples

Plant Total RNA Extraction Kit For Low Polysaccharide And Polyphenol Samples

Description:

The Plant Total RNA Isolation kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from various plant tissues with low polysaccharides and polyphenols content. For plant samples with high polysaccharides or polyphenols content, it is recommended to use Plant Total RNA Isolation Plus Kit to get better RNA extraction results. The kit provides the DNA-Cleaning column that can easily remove genomic DNA from the supernatant and tissue lysate. RNA-only column can effectively bind RNA. The kit can process a large number of samples at the same time.

The entire system does not contain RNase, so the purified RNA will not be degraded. Buffer PRW1 and Buffer PRW2 can ensure that the RNA obtained is not contaminated by protein, DNA, ions, and organic compounds.

Specifications:

50 Preps, 200 Preps

Features&advantages:

-Simple—all operations are completed at room temperature

-Fast—operation can be completed in 30 minutes

-Safe—no organic reagent used

-High quality—the purified RNA is highly pure, free of protein and other impurities, and can meet various downstream experimental applications.

-Particularly suitable for the purification of RNA from plant samples with low polysaccharides and polyphenols content.

-No need to worry about RNA degradation. The whole system is RNase-Free

-Effectively remove DNA using DNA-Cleaning Column

-Remove DNA without adding DNase

Kit components:

*: Please wear gloves and take protective measures during the operation as PRL1 and buffer PRW1 contain irritating chaotropic salts.

Product information

Kit application:

This Plant RNA isolation kits is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with low polysaccharide and polyphenol content.

Work Flow:

DNA-Cleaning Column specifications

RNA-Only Column specifications

1*:The minimum elution system of 50 μl is a reasonably recommended volume given taking into account RNA recovery and concentration. If in order to improve the yield of RNA, can appropriately increase the elution volume ; if in order to increase the concentration of the purified RNA,in the premise of a portion of the RNA yield sacrificed, the elution volume should be reduced appropriately, such as the use of a 30 μl elution system in order to obtain a higher concentration of RNA.

2*: For larger sample sizes, use multiple RNA-Only Columns for RNA purification.

RNA yield and purity

The yield of RNA purified by Plant Total RNA Isolation Kit is related to initial tissue amount, freshness, preservation time and operation. The following is the yield and purity of RNA by using the kit to extract RNA from various animal tissue. In practice, there might be small differences.

RNA can be purified from a variety of plant tissues using the Plant Total RNA Isolation kit Series, and its yield is related to the plant sample itself, the initial sample size, the sample freshness, the sample retention time, and the operation. The following is the yield and purity of RNA used to extract 50mg of various plant samples using the kit, which may differ from this data in practice.

Note: The above data are obtained from the fresh leaves of the plant, if the leaves of the plant are older, or other parts of the plant (such as leaf veins, rhizomes, etc.),or the sample is stored for too long, and the yield of RNA may be lower than the data in the table above.

Storage and Shelf life:

The Plant RNA Isolation kit can be stored for 24 months at room temperature (15-25℃), or 2-8℃ for longer time. Buffer PRL1 can be stored at 4℃ for 1 month after adding β-mercaptoethanol (it is recommended to add it at the same time as the experiment).