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Plant Total RNA Extraction Kit For Low Polysaccharide And Polyphenol Samples

Plant Total RNA Extraction Kit For Low Polysaccharide And Polyphenol Samples

Plant Total RNA Extraction Kit For Low Polysaccharide And Polyphenol Samples
Plant Total RNA Extraction Kit For Low Polysaccharide And Polyphenol Samples
Plant Total RNA Extraction Kit For Low Polysaccharide And Polyphenol Samples
Product Details:
Place of Origin: China
Brand Name: FOREGENE
Certification: ISO CE
Model Number: RE-05011
Payment & Shipping Terms:
Minimum Order Quantity: 1 Kits
Price: 122 USD per box
Packaging Details: Packing with safe trans carton
Delivery Time: 5-8 days
Payment Terms: L/C, D/P, T/T
Supply Ability: 100000 kits per month
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Detailed Product Description
Features: Effectively Remove DNA Using DNA-Cleaning Column Product Name: Plant Total RNA Extraction Kit
Type: For Low Polysaccharide And Polyphenol Samples MOQ: 1 Kits
OEM: 100 Boxes Brand: FOREGENE
Specification: 50 Reactions Delivery Time: 7 Working Days
High Light:

Plant Total RNA Extraction Kit

,

Low Polysaccharide RNA Extraction Kit

Plant Total RNA Extraction Kit For Low Polysaccharide And Polyphenol Samples

 

 

Description:

 

The Plant Total RNA Isolation kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from various plant tissues with low polysaccharides and polyphenols content. For plant samples with high polysaccharides or polyphenols content, it is recommended to use Plant Total RNA Isolation Plus Kit to get better RNA extraction results. The kit provides the DNA-Cleaning column that can easily remove genomic DNA from the supernatant and tissue lysate. RNA-only column can effectively bind RNA. The kit can process a large number of samples at the same time.

The entire system does not contain RNase, so the purified RNA will not be degraded. Buffer PRW1 and Buffer PRW2 can ensure that the RNA obtained is not contaminated by protein, DNA, ions, and organic compounds.

 

 

Specifications:

 

50 Preps, 200 Preps

 

Features&advantages:

-Simple—all operations are completed at room temperature

-Fast—operation can be completed in 30 minutes

-Safe—no organic reagent used

-High quality—the purified RNA is highly pure, free of protein and other impurities, and can meet various downstream experimental applications.

-Particularly suitable for the purification of RNA from plant samples with low polysaccharides and polyphenols content.

-No need to worry about RNA degradation. The whole system is RNase-Free

-Effectively remove DNA using DNA-Cleaning Column

-Remove DNA without adding DNase

 

Kit components:

 

Plant Total RNA Isolation Kit

 

Kit components

RE-05011 RE-05014
50 T 200 T
Buffer PRL1* 25ml 100ml
Buffer PRL2 16.5ml 66ml
Buffer PRW1* 25ml 100ml
Buffer PRW2 24ml 96ml
RNase-Free ddH2O 10ml 40ml
RNA-only Column 50 200
DNA-Cleaning Column 50 200
Instruction Manual 1 piece 1 piece

*: Please wear gloves and take protective measures during the operation as PRL1 and buffer PRW1 contain irritating chaotropic salts.

 

Product information

 

 

Format Spin column Purification component Foregene column, reagent
Flux 1-24 samples Time per prep ~30 min (24 samples)
Centrifuge Desk centrifuge Plant lysate separation Centrifugal separation
Purification column RNA carrying capacity 80μg Samples amount 50 mg
Elution volume 50-200 μL Maximum loading volume

800 μL

 

 

Kit application:

 

This Plant RNA isolation kits is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with low polysaccharide and polyphenol content.

 

 

Work Flow:

 

Plant Total RNA Extraction Kit For Low Polysaccharide And Polyphenol Samples 0

 

 

DNA-Cleaning Column specifications

 

Mechanism Specifically adsorp DNA

Function

Plant Total RNA Extraction Kit For Low Polysaccharide And Polyphenol Samples 1

Remove DNA contamination, Filter and separates the lysate
Maximum loading volume 700μl

 

 

RNA-Only Column specifications

 

Maximum RNA binding capacity 160μg
Maximum loading volume 800μl
RNA size distribution RNA≥200nt
Minimum elution volume 1* 50μl
Selection of samples Fresh young leaves
Maximum amount of starting material 2* 50mg

1*:The minimum elution system of 50 μl is a reasonably recommended volume given taking into account RNA recovery and concentration. If in order to improve the yield of RNA, can appropriately increase the elution volume ; if in order to increase the concentration of the purified RNA,in the premise of a portion of the RNA yield sacrificed, the elution volume should be reduced appropriately, such as the use of a 30 μl elution system in order to obtain a higher concentration of RNA.

2*: For larger sample sizes, use multiple RNA-Only Columns for RNA purification.

 

RNA yield and purity

The yield of RNA purified by Plant Total RNA Isolation Kit is related to initial tissue amount, freshness, preservation time and operation. The following is the yield and purity of RNA by using the kit to extract RNA from various animal tissue. In practice, there might be small differences.

 

RNA can be purified from a variety of plant tissues using the Plant Total RNA Isolation kit Series, and its yield is related to the plant sample itself, the initial sample size, the sample freshness, the sample retention time, and the operation. The following is the yield and purity of RNA used to extract 50mg of various plant samples using the kit, which may differ from this data in practice.

Fresh young leaves 50 mg RNA yield (μg) OD260/280 OD260/230
Tobacco 27-30 1.8-2.1 1.8-2.1
Tomato 32-35 1.8-2.1 1.8-2.1
Soybean 31-34 1.8-2.1 1.8-2.1
Corn 15-17 1.8-2.1 1.8-2.1
Rape 8-10 1.8-2.1 1.8-2.0
Rice 15-17 1.8-2.1 1.8-2.1

 

Note: The above data are obtained from the fresh leaves of the plant, if the leaves of the plant are older, or other parts of the plant (such as leaf veins, rhizomes, etc.),or the sample is stored for too long, and the yield of RNA may be lower than the data in the table above.

 

Storage and Shelf life:

 

The Plant RNA Isolation kit can be stored for 24 months at room temperature (15-25℃), or 2-8℃ for longer time. Buffer PRL1 can be stored at 4℃ for 1 month after adding β-mercaptoethanol (it is recommended to add it at the same time as the experiment).

 

Contact Details
Foregene Co., Ltd.

Contact Person: David

Tel: +8616602829025

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