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Foreasy Taq DNA Polymerase HotStar Taq Polymerase Kit For PCR/QPCR

Foreasy Taq DNA Polymerase HotStar Taq Polymerase Kit For PCR/QPCR

  • Foreasy Taq DNA Polymerase HotStar Taq Polymerase Kit For PCR/QPCR
  • Foreasy Taq DNA Polymerase HotStar Taq Polymerase Kit For PCR/QPCR
  • Foreasy Taq DNA Polymerase HotStar Taq Polymerase Kit For PCR/QPCR
Foreasy Taq DNA Polymerase HotStar Taq Polymerase Kit For PCR/QPCR
Product Details:
Place of Origin: China
Brand Name: Foreasy
Certification: ISO
Model Number: IM-01011
Payment & Shipping Terms:
Minimum Order Quantity: 5 KU
Price: 0.075 usd/u
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 10000 ku per month
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Detailed Product Description
Brnad: Foreasy Specifications: 1.7ml ×3, 1.7ml ×6 , 1.7ml ×24
Type: Taq DNA Polymerase Applicable Samples: PCR Amplification Of DNA Fragments,DNA Labeling,sequencing, PCR Plus A Tail
Product Name: Foreasy Taq DNA Polymerase MOQ: 5 KU
High Light:

Taq Polymerase Kit For PCR

,

1.7ml Taq DNA Polymerase

,

QPCR Taq Polymerase Kit

Foreasy Taq DNA Polymerase HotStar Taq Polymerase Kit for PCR/qPCR

Product Introduction

Foreasy Taq DNA Polymerase is a new Taq enzyme expressed in Escherichia coli engineering bacteria by gene recombination technology. The enzyme itself has a certain hot-start activity and can be used for conventional PCR and qPCR; it has 5'→3' DNA polymerase activity and 5'→3' exonuclease activity, but no 3'→5' exonuclease activity.

Foreasy Taq DNA Polymerase HotStar Taq Polymerase Kit For PCR/QPCR 0

Component

Component

IM-01011

IM-01012

IM-01013

Foreasy Taq DNA Polymerase

(5 U/μL)

 

5000 U (1 mL)

 

50 KU (10 mL)

 

500 KU (100 mL)

2× Taq Reaction Buffer

 

25 mL ×5

 

250 mL ×5

 

500 mL ×25

 

Storage

-20 ± 5 °C for 2 years or at -80 °C for long-term storage.

 

Features

High specificity: The enzyme has a certain hot-start activity.

Fast Amplification: 10 sec/kb .

Highly adaptable template: can be used to efficiently amplify GC High value, various difficult-to-amplify DNA template.

Strong fidelity: ordinary Taq Enzyme 6 times.

Strong thermal stability: It can be placed at 37 °C for a week and maintains more than 90% activity

 

Application

Various PCR/qPCR systems and direct PCR systems

PCR amplification of DNA fragments

DNA labeling

DNA sequencing

PCR A-tailed

 

U Definition

1U: The amount of enzyme required to incorporate 10 nmol of deoxynucleotides into acid-insoluble matter using activated salmon sperm DNA as template/primer for 30 minutes at 74°C.

 

Activity Assay Conditions

1× Taq Reaction Buffer,1.5 mM MgCl2;0.2 mg/mL activated calf thymus DNA,0.2 mM dNTPs.

Storage

20 mM Tris-HCl (pH 8.0),1 mM DTT,0.1 mM EDTA,50% glycerol,stabilizer.

2× Taq Reaction Buffer

Contains optimized ratios of Tris, KCl, MgCl2 and other ingredients.

Instance Reaction Protocol

 

Template DNA

X μL

dNTPs (10 mM each)

1 μL

Primer-F

1 μL

Primer-R

1 μL

ddH2O

To 50 μL

Total Volume

50 μL

 

 

 

Reaction Condition

Temperature Time Cycle
37°C 5mins 1
94°C 5mins 1
94°C 10 Secs

 

35

60°C 10 Secs
72°C 20 sec/kb
72°C 2mins 1

 

 

Note: For 10 µL and 20 µL systems, add an equal volume of mineral oil if the thermal cycler does not have a heated lid.

PCR reaction conditions vary depending on the structural conditions of templates, primers, etc. In the specific operation ,it is necessary to design the optimal reaction conditions including the annealing temperature, extension time and so on , which is according to the different template type, the size of the target fragment, the base sequence of the amplified fragment, and the GC content and length of the primer.

Contact Details
Foregene Co., Ltd.

Contact Person: David

Tel: +8616602829025

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