Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
 |
| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | TP-04211/04213 |
| Minimum Order Quantity: | 1kit |
|---|---|
| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Product Name: | Blood SuperDirect PCR Kits With Heparin |
|---|---|---|---|
| Function: | Extraction | Type: | Blood Direct PCR |
| Application: | Dedicated To Direct PCR Identification Of Heparin Anti-coagulated Whole Blood | Operation Temperature: | Room Temperature |
| High Light: | Direct PCR Kits With Heparin,1.5ml Blood Direct PCR Kits |
||
Strong amplification ability PCR system Blood SuperDirect PCR Kits With Heparin no pretreatment
Description
This product uses a unique PCR buffer system, which allows direct PCR with whole blood samples as a template without DNA extraction or sample processing. EDTA, Heparin anticoagulated whole blood and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification and identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can be up to 45% of the PCR system and 20% of mouse blood, which can sensitively amplify blood samples. Genomic and foreign target DNA fragments.
2× SuperEasyTM Mix-Heparin contains our company’s unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhncer, optimizer and stabilizer.
Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
According to the different anticoagulants used, this product is divided into two categories: EDTA anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-Heparin); You can also choose the corresponding kit according to the needs of the experiment, see the selection of kit type for details.
Kit conponents
|
Blood SuperDirectTM PCR Kit-Heparin Heparin Anticoagulated whole blood direct PCR kit |
||||
| Kit composition | TP-0421T | TP-04211 | TP-04212 | TP-04213 |
| 50 T | 200 T | 500 T | 2000 T | |
| 2× SuperEasyTM Mix-Heparin | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 |
| Manual | 1 | 1 | 1 | 1 |
Feature
- No need for time-consuming and expensive DNA purification, no pretreatment, and direct use of whole blood as a PCR template.
- The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.
- 2× SuperEasyTM Mix-Heparin High blood tolerance: up to 45% in human blood and 20% in mouse blood.
- The sample is fully enclosed operation, so there is no need to worry about sample contamination and false positive PCR results.
Transportation
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.
Storage
This kit should be stored at -20℃.
- 2× SuperEasyTM Mix-Heparin,If used frequently, it can be stored at 4°C for short-term storage (use up within 10 days).
- 6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.
Electropherogram
The blood direct PCR series kit can amplify the target gene from a variety of sources of anticoagulated whole blood as a template. 2× SuperEasyTM Mix-Heparin has strong tolerance to PCR reaction inhibitors, high detection sensitivity and strong amplification specificity. Use human blood as a template to amplify related genes and analyze by 1% agarose gel electrophoresis, as shown in the figure below:
Note:(Please read carefully before using the kit)
- Please try to use newly taken anticoagulant blood samples for related experiments; if it is a frozen sample, avoid repeated freezing and thawing, otherwise the DNA fragment used as the PCR template will be small and the PCR efficiency will be affected.
- Pay attention to the cleanliness of experimental equipment and experimental operation methods to avoid cross-contamination between samples.
- 2× SuperEasyTM Mix-Heparin Avoid repeated freezing and thawing, otherwise it will affect the PCR efficiency
- If the ambient temperature is high, 2× SuperEasyTM Mix-Heparin may become turbid and can be placed on ice1-2min, wait for the solution to clarify, and mix it upside down for 3-5 times before using.
- During electrophoresis detection, do not use Loading Buffer containing SDS, otherwise a large group of tailing bright bands will appear in the lanes during electrophoresis, which will affect the experimental results.
Work Flow
Contact Person: Maggie
Tel: +8615281067355
