Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | TP-04121/04123 |
| Minimum Order Quantity: | 1 kit |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Product Name: | Blood SuperDirect PCR Kits(UNG) With EDTA |
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| Function: | Extraction | Type: | Blood Direct PCR |
| Application: | EDTA Anticoagulated Whole Blood | Operation Temperature: | Room Temperature |
| High Light: | Blood Direct PCR Kits UNG,EDTA Anticoagulated Blood Direct PCR Kits |
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Molecular biology lab Blood Super Direct PCR Kits UNG with strong amplification ability
Description
This product uses a unique PCR buffer system, which allows direct PCR with whole blood samples without DNA extraction or sample processing. EDTA, Heparin anticoagulated whole blood, and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can reach 45% of the PCR system, and that of mouse blood can reach 20%, which can sensitively amplify blood samples. Genomic and exogenous DNA fragments of interest.
2× SuperEasyTM Mix-EDTA contains our company's unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhancer, optimizer and stabilizer.
2× SuperEasyTM Mix (UNG) replaces dTTP with dUTP on the basis of 2× SuperEasyTM Mix, and at the same time adds a UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP. Before the PCR reaction, the PCR product containing uracil is degraded by UNG enzyme. UNG enzyme will not have any effect on the template that does not contain uracil, so as to ensure the specificity and accuracy of amplification and prevent the possibility of large-scale genetic testing. PCR product contamination problems arise.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions.
D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems. The amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
According to the different anticoagulants used, the products are divided into two categories: EDTA anticoagulation whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulation whole blood direct PCR kit series (BloodSuperDirectTM PCR Kit series). -Heparin); the corresponding kit can also be selected according to the experimental needs, see the selection of kit types for details.
Kit conponents
| Blood SuoerDirectTM PCR Kit(UNG)-EDTA | ||||
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Kit composition |
TP-0412T | TP-04121 | TP-04122 | TP-04123 |
| 50 T | 200 T | 500 T | 2000 T | |
| 2× SuperEasyTM Mix(UNG)-EDTA | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 |
| Manual | 1 | 1 | 1 | 1 |
Feature
- No time-consuming and expensive DNA purification, no pretreatment, and whole blood as a PCR template.
- The system has strong amplification ability and can sensitively detect genomic and exogenous target DNA fragments in blood.
- 2× SuperEasyTM Mix(UNG)-EDTA High blood tolerance: up to 45% in human blood and 20% in mouse blood.
- The sample is completely closed, and there is no need to worry about sample contamination and false positive PCR results. Anti-pollution PCR system 2×SuperEasyTM Mix(UNG)-EDTA can effectively eliminate the pollution caused by PCR products and ensure the specificity and accuracy of amplification.
Application
Dedicated to direct PCR identification of EDTA anticoagulated whole blood. (Including: human blood, mouse blood, chicken blood, bird blood, cow blood, dog blood, etc.).
Transportation
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.
Storage
This kit is stored at -20°C.
- 2× SuperEasyTM Mix(UNG)-EDTA,If used frequently, it can also be stored at 4°C for short-term storage (use up within 10 days).
- 6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.
Notes:(Please read carefully before using the kit)
- Please try to use freshly collected anticoagulant samples for relevant experiments; if the samples are frozen, avoid repeated freezing and thawing, otherwise the DNA fragments used as PCR templates will be smaller, which will affect the PCR efficiency.
- Pay attention to the cleaning of laboratory utensils and the operation methods of the experiment to avoid cross-contamination between samples.
- 2× SuperEasyTM Mix(UNG)-EDTA should avoid repeated freezing and thawing, otherwise it will affect the PCR efficiency
- If the ambient temperature is high, the 2× SuperEasyTM Mix(UNG)-EDTA may become turbid. It can be placed on ice for 1-2 minutes. When the solution is clear, invert and mix for 3-5 times before use.
Do not use Loading Buffer containing SDS for electrophoresis detection, otherwise a large group of trailing bright bands will appear in the swimming lane during electrophoresis, which will affect the experimental results.
Work Flow
Contact Person: Maggie
Tel: +8615281067355
