Spin Column Total RNA Isolation Kit Plus HIGH In Polysaccharide And Polyphenol RNA Isolation Kits Spin Column

Spin column third generation RNA isolation Plant total RNA Isolation kit Plus HIGH in polysaccharide and polyphenol

Description:

The Plant Total RNA Isolation kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from various plant tissues with low polysaccharides and polyphenols content. For plant samples with high polysaccharides or polyphenols content, it is recommended to use Plant Total RNA Isolation Plus Kit to get better RNA extraction results. The kit provides the DNA-Cleaning column that can easily remove genomic DNA from the supernatant and tissue lysate. RNA-only column can effectively bind RNA. The kit can process a large number of samples at the same time.

The entire system does not contain RNase, so the purified RNA will not be degraded. Buffer PRW1 and Buffer PRW2 can ensure that the RNA obtained is not contaminated by protein, DNA, ions, and organic compounds.

Specifications:

50 Preps, 200 Preps

Kit components:

*: Buffer PSL1, PRW1 contains irritating base salts, please pay attention to gloves and carry out relevant protective measures when operating.

Product information

Features&advantages:

Kit application:

This Plant RNA isolation kits is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with high polysaccharide and polyphenol content.

Work Flow:

Storage and Shelf life:

The Plant RNA Isolation kit Plus can be stored for 24 months at room temperature (15-25℃), or 2-8℃ for longer time. Buffer PRL1 can be stored at 4℃ for 1 month after adding β-mercaptoethanol (it is recommended to add it at the same time as the experiment).

Kit component information

Buffer Buffer PSL1: Provides the environment required for plant grinding tissue cleavage.

Buffer PS: Supplementary polysaccharide polyphenol content of plant tissue cleavage reactions required for the environment.

Buffer Buffer PSL2: Provides a specific upper column environment for RNA.

Buffer PRW1: Removes impurities such as protein, DNA in RNA.

Buffer PRW2: Removal of salt ions remaining in RNA.

RNASE-Free DDH₂O: Total RNA on the purified column membrane.

DNA-CLEANING COLUMN: DNA in the tissue lysis of tissue, and filtered to remove solid impurities in the lysis product.

RNA-ONLY Column: Specifically adsorbed with total RNA in DNA-CLEANING Column filtrate.

DNA-Cleaning Column specifications

RNA-Only Column specifications

1*:The minimum elution system of 50 μl is a reasonably recommended volume given taking into account RNA recovery and concentration. If in order to improve the yield of RNA, can appropriately increase the elution volume ; if in order to increase the concentration of the purified RNA,in the premise of a portion of the RNA yield sacrificed, the elution volume should be reduced appropriately, such as the use of a 30 μl elution system in order to obtain a higher concentration of RNA.

2*: For larger sample sizes, use multiple RNA-Only Columns for RNA purification.

RNA yield and purity

The yield of RNA purified by Plant Total RNA Isolation Kit Plus is related to initial tissue amount, freshness, preservation time and operation. The following is the yield and purity of RNA by using the kit to extract RNA from various animal tissue. In practice, there might be small differences.

RNA can be purified from a variety of plant tissues using the Plant Total RNA Isolation kit Series, and its yield is related to the plant sample itself, the initial sample size, the sample freshness, the sample retention time, and the operation. The following is the yield and purity of RNA used to extract 50mg of various plant samples using the kit, which may differ from this data in practice.

Note: The above data are obtained from the fresh leaves of the plant, if the leaves of the plant are older, or other parts of the plant (such as leaf veins, rhizomes, etc.),or the sample is stored for too long, and the yield of RNA may be lower than the data in the table above.