Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 50T, 100T, 250T |
| Minimum Order Quantity: | 1 kit |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Product Name: | Laboratory Research Use Bacterial Genomic DNA Isolation Kit 5000xg Spin Column |
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| CAT NO.: | DE-05311/05312/05313 | Type: | DNA-Only Column |
| Application: | Purifying Genomic DNA | Operation Temperature: | Room Temperature |
| High Light: | CE Bacterial DNA Isolation Kit,Laboratory Research Bacterial DNA Isolation Kit |
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No RNase contamination Laboratory reagent Bacterial DNA Isolation kit DNA Isolation Kit
Description
This kit provides a quick and easy method of extracting genomic DNA from cultures of bacteria (Gram-negative and Gram-positive) from various sources; it can process less than 3ml of bacterial cultures in the logarithmic growth phase (1×109 bacteria). The kit is matched with high-efficiency Foregene Protease, which allows 15-50μg of high-quality genomic DNA to be extracted within 1 hour. In addition, the kit can also extract genetic material other than the genome, such as plasmids, Cosmid, BAC, etc.
The DNA-only silica gel matrix material used in the spin column is a unique new material of the company, which can efficiently and specifically adsorb DNA. The maximum adsorption capacity for DNA is 80μg. The unique buffer and elution system can maximize the removal of RNA, Impurity proteins, ions and other organic compounds in cells. The extracted genomic DNA fragment is large, high purity, stable and reliable quality, and the size of the DNA fragment is stable at about 23kb.
Kit conponents
| Bacterial DNA Isolation Kit | |||
| Kit contents | DE-05311 | DE-05312 | DE-05313 |
| 50 T | 100 T | 250 T | |
| Buffer ML1 | 25ml | 50ml | 125ml |
| Buffer ML2 | 25ml | 50ml | 125ml |
| Buffer PW | 30ml | 60ml | 150ml |
| Buffer WB | 25ml | 50ml | 125ml |
| Buffer EB | 10ml | 20ml | 50ml |
| Foregene Protease | 1.25ml | 1.25ml x2 | 6.25ml |
| Lysozyme | 250mg | 500mg | 500mg x2 |
| Buffer TE | 5ml | 8ml | 10ml |
| DNA-Only Column | 50 sets | 100 sets | 250 sets |
| IFU | 1 piece | 1 piece | 1 piece |
Features&advantages
- No RNase contamination: No need to add RNase to remove the RNA in the genomic DNA, avoiding the laboratory from being contaminated by exogenous RNase.
- Fast speed: Foregene Protease has higher activity than similar proteases and can digest broken bacterial samples in a short time.
- Convenience: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
- Safety: no organic reagent extraction is required.
- High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Genomic DNA Application
The genomic DNA purified by the Bacterial DNA isolation kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern hybridization, library construction and other experiments.
Quality Control
According to FOREGENE’s Total Quality Management System , each batch of Bacterial DNA isolation kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
DNA-Only Column Characters
| Maximum binding capacity | 80μg |
| Maximum loading volume | 800μl |
| Longset DNA fragment | 23kb |
| Minimum elution volume* | 100μl |
| Selection of samples | Cultivate negative bacteria for 16-20hr |
| Maximum amount of starting material* | 3ml |
*: The minimum elution system of 100μl is a reasonable recommended volume considering the DNA recovery rate and concentration. If you want to increase the yield of DNA, you can increase the volume of the eluate; if you want to increase the concentration of purified DNA, at the expense of part of the DNA yield, reduce the volume of the eluate appropriately, such as using a 50μl elution system. In order to get a higher concentration of DNA.
Genomic DNA extraction yield and purity
The amount of genomic DNA obtained by using the Bacterial DNA isolation kit to process bacteria is related to the source of the sample, storage time, culture time and other factors. Generally, the bacterial genomic DNA obtained under normal circumstances is the sum of the DNA in the bacterial cell, and the genomic DNA yield of the bacteria cultured overnight in 3ml is about 15-50μg. In actual operation, the amount obtained may differ slightly from the data.
Storage and Shelf life
- This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to put the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
-Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months); when stored at 4°C, its activity and stability will be better, so it is recommended to store it at 4°C, remember not to keep it at -20°C.
-Lysozyme is a dry powder, which is active when stored at 2-8°C for a long time (3 months). For longer storage, please store it at -20°C.
Contact Person: Maggie
Tel: +8615281067355
