Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 50T, 100T, 250T |
| Minimum Order Quantity: | 1 kit |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Product Name: | Higy DNA Yield Blood DNA Midi Kit DNA Extraction Kits |
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| CAT NO.: | DE-05111, DE-05112, DE-05113 | Type: | DNA-Only Column |
| Application: | Extraction And Purification Of Genomic DNA | Operation Temperature: | Room Temperature |
| High Light: | Genomic DNA Purification Kit,Lab Reagent DNA Isolation Kits,DE-05113 DNA Midi Kit |
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Safe and fast lab reagent Blood DNA Midi Kit DNA Isolation Kits for genomic DNA purification
Description
The blood sample contains a large number of red blood cells and platelet cells, which makes the blood processing more troublesome. However, the blood genomic DNA extracted by the general method is not high in purity and the content is too low. This kit uses the brand-new Foregene Protease Plus and unique BL1 and BL2 buffer systems, which can completely digest anticoagulated blood and coagulation samples in the shortest time, thereby avoiding DNA degradation to the greatest extent and obtaining the largest amount of genomic DNA. The fast blood processing system and simple spin column operation greatly simplify the extraction of blood genome, so that high-quality, high-purity genomic DNA can be obtained within 40 minutes.
The DNA-only silica gel membrane used in the spin column of this kit is a unique new material of the company, which efficiently and specifically adsorbs DNA, and can remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The obtained DNA fragments are large, with high purity, stable and reliable quality, and the maximum carrying capacity of a spin column is 80 μg DNA. The obtained DNA can be used for molecular biology experiments such as restriction digestion, PCR, Southern hybridization, library construction and so on. The kit can process up to 5ml of blood at a time to obtain a sufficient amount of high-purity genomic DNA.
Kit conponents
| Blood DNA Midi Kit | |||
| Kit contents | DE-05121 | DE-05122 | DE-05123 |
| 50 T | 100 T | 250 T | |
| Buffer BL1 | 25ml | 50ml | 125ml |
| Buffer BL2 | 25ml | 50ml | 125ml |
| Buffer DC | 500ml | 500ml x2 | 500ml x5 |
| Buffer PW | 25ml | 50ml | 125ml |
| Buffer WB1 | 15ml | 30ml | 75ml |
| Buffer EB | 10ml | 20ml | 50ml |
| Foregene Protease Plus | 2.5ml | 5ml | 12.5ml |
| DNA-Only Column | 50 sets | 100 sets | 250 sets |
| IFU | 1 piece | 1 piece | 1 piece |
Genomic DNA Application
The genomic DNA purified by the Blood DNA Midi Kit has high purity and can be used for routine molecular biology operations, such as restriction enzyme digestion, PCR, Southern hybridization, library construction and other experiments.
Quality Control
According to FOREGEN’s Total Quality Management System, each batch of Blood DNA Midi kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
Features&advantages
- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
- Fast speed: Foregene Protease Plus has higher activity than similar proteases, and digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.
- Convenience: The centrifugation is performed at room temperature, 4°C centrifugation or ethanol precipitation of DNA is not required.
- Safety: No organic reagent extraction is used.
- High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
DNA-Only Column Characters
| Maximum binding capacity | 80μg |
| Maximum loading volume | 800μl |
| Longset DNA fragment | 23kb |
| Minimum elution volume* | 100μl |
| Selection of samples | Fresh anticoagulated whole blood |
| Maximum amount of starting material* | 5ml |
*: The minimum elution system of 100μl is a reasonable recommended volume considering the DNA recovery rate and concentration. If you want to increase the yield of DNA, you can increase the volume of the eluate, if you want to increase the concentration of the purified DNA, you can reduce the volume of the eluate appropriately, such as using a 50μl elution system, while sacrificing part of the DNA yield. In order to get a higher concentration of DNA.
Genomic DNA extraction yield and purity
The extraction yield of blood genomic DNA is related to the tissue source, storage conditions, storage time, dosage and other factors. The purity of the obtained genomic DNA meets the routine molecular biology experiment operation, and OD260/280 is between 1.7-1.9.
Genomic DNA fragment size
The Blood DNA Midi kit uses silica membrane columns to isolate and purify genomic DNA from a variety of sample sources. The purified genomic DNA fragments size are all around 23 kb.
Storage and Shelf life
Blood DNA Midi Kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
The Foregene Protease Plus solution has a unique formula, which is active when stored at room temperature for a long time (3 months); it will be more active and stable when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.
Contact Person: Maggie
Tel: +8615281067355
