Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 50T, 100T, 250T |
| Minimum Order Quantity: | 1 kit |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Product Name: | Safe And Fast Genomic DNA Purification Lab Reagent Blood DNA Mini Kit DNA Isolation Kits |
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| CAT NO.: | DE-05111, DE-05112, DE-05113 | Type: | DNA-Only Column |
| Application: | Extraction And Purification Of Fresh Or Frozen Anticoagulant Whole Blood Genomic DNA | Operation Temperature: | Room Temperature |
| High Light: | CE Blood DNA Isolation Kits,Spin Column Blood DNA Mini Kit |
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Safe and fast genomic DNA purification lab reagent Blood DNA Mini Kit DNA Isolation Kits
Description:
This kit adopts new Foregene Protease Plus and unique BL1 and BL2 buffer systems, which can completely digest anticoagulant blood samples in a short period of time, thus avoiding DNA degradation to the maximum degree and obtaining the maximum amount of genomic DNA.The rapid blood processing system and the simple DNA-Only column operation greatly simplify the extraction of blood genome, making the genomic DNA can be obtained of high quality and high purity within 40 minutes.
The DNA-only silica gel membrane used in the centrifuge column of this kit is a new type of material unique to our company, which can bind DNA efficiently and specifically, and can remove RNA, impurities, proteins, ions and other organic compounds in cells to the maximum extent.The obtained DNA fragments are large, high purity, stable and reliable quality. The maximum carrying capacity of a DNA-Only column is 80μg DNA.The obtained DNA can be used in molecular biology experiments such as enzyme digestion, PCR, Southern hybridization and library construction.
The kit can process up to 1ml of blood at a time to obtain a sufficient amount of high-purity genomic DNA.
Kit conponents
| Blood DNA Mini Kit | |||
| Kit component | DE-05111 | DE-05112 | DE-05113 |
| 50T | 100T | 250T | |
| Buffer BL1 | 15ml | 30ml | 75ml |
| Buffer BL2 | 15ml | 30ml | 75ml |
| Buffer DC | 100ml | 200ml | 500ml |
| Buffer PW | 25ml | 50ml | 125ml |
| Buffer WB1 | 15ml | 30ml | 75ml |
| Buffer EB | 10ml | 20ml | 50ml |
| Foregene Protease Plus | 1.25ml | 2.5ml | 6.5ml |
| DNA-Only Column | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
*: Buffer BL2 and Buffer PW contain irritating liquid salt. Please wear gloves and take relevant protective measures when operating.
Product information
| Model | Spin-column type | Purification components | Foregene spin column,reagent |
| Throughput | 1-24 samples | Preparation time | 40-50min(24 samples) |
| Centrifuge | Table centrifuge | Sample enzymatic hydrolysate separation | Centrifugal separation |
| Purification column DNA load carrying capacity | 800μl | Purification column DNA loading capacity | 80μg |
| Elution volume | 100-200μl | Blood sample throughput | 50-1000μl |
Features&advantages
- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
- Fast speed: Foregene Protease Plus has higher activity than similar proteases, and digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.
- Convenience: The centrifugation is performed at room temperature, 4°C centrifugation or ethanol precipitation of DNA is not required.
- Safety: No organic reagent extraction is used.
- High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Applications of genomic DNA:
The genomic DNA purified by the Blood DNA Mini Kit is of high purity and can be used for routine molecular biology operations, such as:
Enzyme digestion, PCR, Southern hybridization, library construction and other experiments.
Genomic DNA extraction yield and purity:
The extraction yield of blood genomic DNA is related to the source of tissue, storage conditions, storage time, dosage and other factors.
The purity of the obtained genomic DNA meets the routine molecular biology experimental operation, and its OD260/280 is between 1.7-1.9.
Genomic DNA Fragment Size:
The blood genomic DNA extraction kit uses silica membrane column to separate and purify genomic DNA from various sample sources.
The size of the genomic DNA fragments obtained by chemistry was around 23kb.
Storage and Shelf life:
This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
The Foregene Protease Plus solution has a unique formula, which is active when stored at room temperature for a long time (3 months); it will be more active and stable when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.
Contact Person: Maggie
Tel: +8615281067355
