Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 50T, 100T, 250T |
| Minimum Order Quantity: | 100kits |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Product Name: | Laboratory Research Reagent Blood DNA Extraction Kit High Purity Spin Column |
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| CAT NO.: | DE-05111, DE-05112, DE-05113 | Type: | DNA-Only Column |
| Application: | Whole Blood Genomic DNA | Operation Temperature: | Room Temperature |
| High Light: | Research Reagent Blood DNA Extraction Kit,Spin Column DNA Extraction Kit |
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Laboratory research reagent Blood DNA Extraction Kit high purity spin column
Description
Blood DNA midi kit adopts new Foregene Protease Plus and unique BL1 and BL2 buffer systems, which can completely digest anticoagulant blood samples in a short period of time, thus avoiding DNA degradation to the maximum degree and obtaining the maximum amount of genomic DNA.The rapid blood processing system and the simple centrifugal column operation greatly simplify the extraction of blood genome, making the genomic DNA of high quality and high purity can be obtained within 40 minutes.
The DNA-only silica gel membrane used in the centrifuge column of this kit is a new type of material unique to our company, which can adsorb DNA efficiently and specifically, and can remove RNA, impurities, proteins, ions and other organic compounds in cells to the maximum extent.The obtained DNA fragments are large, high purity, stable and reliable quality. The maximum carrying capacity of a centrifugal column is 80μg DNA.The obtained DNA can be used in molecular biology experiments such as enzyme digestion, PCR, Southern hybridization and library construction.
The kit can process up to 1ml of blood at a time to obtain a sufficient amount of high-purity genomic DNA.
Kit conponents
| Blood DNA Midi Kit | |||
| Kit contents | DE-05121 | DE-05122 | DE-05123 |
| 50 T | 100 T | 250 T | |
| Buffer BL1 | 25ml | 50ml | 125ml |
| Buffer BL2 | 25ml | 50ml | 125ml |
| Buffer DC | 500ml | 500ml x2 | 500ml x5 |
| Buffer PW | 25ml | 50ml | 125ml |
| Buffer WB1 | 15ml | 30ml | 75ml |
| Buffer EB | 10ml | 20ml | 50ml |
| Foregene Protease Plus | 2.5ml | 5ml | 12.5ml |
| DNA-Only Column | 50 sets | 100 sets | 250 sets |
| IFU | 1 piece | 1 piece | 1 piece |
Product INFO
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time |
40-50min |
| Centrifuge | Table centrifuge | Sample enzymatic hydrolysate separation | centrifugal separation |
| Purification column DNA load carrying capacity | 80μg | Liquid volume of spin column | 800μl |
| Elution volume | 100-200μl | Blood sample processing volume |
1-5ml |
Features&advantages
| No RNase contamination | The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase. |
| Fast speed | Foregene Protease Plus has higher activity than similar proteases, and digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes. |
| Convenience | The centrifugation is performed at room temperature, 4°C centrifugation or ethanol precipitation of DNA is not required. |
| Safety | No organic reagent extraction is used. |
| High quality | The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments. |
Kit application
Blood DNA midi kit is suitable for extraction and purification of genomic DNA from fresh or frozen anticoagulated whole blood.
Kit components
◆ Buffer DC: Lyses red blood cells in the blood.
◆ Buffer BL1: Provide sample enzymolysis environment.
◆ Foregene Protease Plus: Enzymatically hydrolyze blood samples in the environment of Buffer BL1.
◆ Buffer BL2: Inactivate Foregene Protease Plus and provide a DNA loading environment.
◆ Buffer PW: Remove impurities such as protein and RNA in DNA.
◆ Buffer WB1: Remove residual salt ions in DNA.
◆ Buffer EB: elute the DNA on the purification column membrane.
◆ DNA-Only Column: Specifically adsorb genomic DNA in the lysate.
Quality Control
According to FOREGEN’s Total Quality Management System, each batch of Blood DNA Midi kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
Workflow
Storage and Shelf life
Blood DNA midi kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
The Foregene Protease Plus solution has a unique formula, which is active when stored at room temperature for a long time (3 months); it will be more active and stable when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.
Contact Person: Maggie
Tel: +8615281067355
