Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 5T, 50T, 100T, 200T |
| Minimum Order Quantity: | 1 BOX |
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| Price: | Negotiate |
| Delivery Time: | 1-7 days |
| Payment Terms: | T/T, L/C, Western Union |
| Supply Ability: | 10000 BOX per Month |
| Product Name: | Spin Column Animal MiRNA Isolation Kits RE-0101T For Laboratory Research Use | CAS No.: | RE-0101T, RE-01011, RE-01012, RE-01014 |
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| Application: | Animal Tissues Or Cells | Classification: | General Reagents |
| Purity: | 99.9% | Shelf Life: | 1-2 Years |
| High Light: | Animal MiRNA Isolation Kits,Spin Column MiRNA Isolation Kits |
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spin column Animal MiRNA Isolation Kits RE-0101T for laboratory research use
Description
Animal MiRNA Isolation Kit use the spin column and formula developed by our company, which can efficiently extract small fragments of RNA such as 20-200nt miRNA, siRNA, snRNA from various animal tissues and cells. The kit provides DNA-Cleaning Column, which can easily separate the supernatant from the tissue lysate and remove DNA and long RNA from the sample, which is simple and time-saving; RNA-only Column can efficiently bind RNA with a unique formula , Can process a large number of samples at the same time. The whole system is RNase-Free, so that the purified miRNA is not degraded; Buffer miRW1, Buffer miRW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ions, and organic compound pollution.
Kit components
| Animal miRNA Isolation Kits | ||||
| Kit contents | RE-0101T | RE-01011 | RE-01012 | RE-01014 |
| 5 T | 50 T | 100 T | 200 T | |
| Buffer miRL* | 3ml | 25ml | 50ml | 100ml |
| Buffer miRW1 | 4ml | 35ml | 70ml | 140ml |
| Buffer miRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-Free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| RNA-only Column | 5 sets | 50 sets | 100 sets | 200 sets |
| DNA-Cleaning Column | 5 sets | 50 sets | 100 sets | 200 sets |
| IFU | 1 piece | 1 piece | 1 piece | 1 piece |
Features&advantages
| Room temperature (15-25°C) operation | without ice bath and low-temperature centrifugation |
| RNase-Free | no need to worry about RNA degradation. |
| DNA-Cleaning Column specifically binds DNA | the Animal miRNA Isolation kit can remove genomic DNA contamination without adding additional DNase. |
| High RNA yield | RNA-only Column and unique formula can efficiently purify RNA. |
| Fast speed | The operation is simple and can be completed within 30 minutes. |
| Safety | No organic reagent extraction is required. |
| High quality | The extracted RNA fragments are of high purity, free of protein and other impurities pollution, and can meet the requirements of downstream. |
| Applications | Various experimental applications |
Kit application
Animal MiRNA Isolation Kit is suitable for the extraction and purification of miRNA from a variety of fresh or frozen animal tissues or cells.
The miRNA purified by Animal miRNA isolation kit can be used in various downstream molecular experiments, such as: RT-PCR, Real Time PCR, Northern Blot, chip analysis, molecular cloning, etc.
It is recommended to use RNase-Free ddH2O to elute RNA for immediate use in downstream experiments or store at -80°C. Under the storage condition of -80℃, RNA can be stored for one year.
Storage and Shelf life
Animal MiRNA Isolation Kit can be stored for 24 months under dry conditions at room temperature (15-25°C), if it needs to be stored for a longer period of time, it can be stored at 2-8°C. Buffer miRL can be placed at 4°C for 1 month after adding β-mercaptoethanol (it is recommended to add it now).
Product INFO
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time | ~30min(24 samples) |
| Centrifuge | Table centrifuge |
Tissue enzymolysis separation |
centrifugal separation |
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Purification column RNA load carrying capacity |
80μg |
Liquid volume of spin column |
800μl |
| Elution volume | 50-200μl |
Tissue sample processing volume |
Tissue≤30mg Cell≤5×106 |
According to FOREGENE’s Total Quality Management System, each batch of Animal miRNA isolation kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
Work Flow
Contact Person: Maggie
Tel: +8615281067355
