Buffer NP Super Direct PCR Kit Mouse Tail Direct Kit PCR-UNG TP-01311 TP-01313

Quick and easy Mouse Tail Super Direct PCR Kit one step room temperature

Product Descprition

This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tail and mouse ear samples for PCR reaction, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at room temperature (20-25°C). No heating is required, and other processes such as protein and RNA removal are not required. The released trace amounts of DNA can be used as Template for PCR reaction.

2×M-PCR Easy^TM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, reaction buffer, PCR optimizer and stabilizer.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Product components:

Features&advantages:

• No time-consuming and expensive DNA purification is required.
• The sample demand is small, just take mouse tails (or mouse ears with a diameter of 2-5mm) above 2mm.
• Easy to operate, it can be lysed without heating to inactivate the protease, and the lysed product can be directly subjected to PCR reaction.
• Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) can be used to extract genomic DNA from the processed rat tail.
• Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

Kit parameters:

Application: Mouse tail, mouse ears, muscle and other tissues.

DNA released from sample lysis: only used as a PCR template.

The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

Product quality control:

In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of Mouse Tail Super Direct PCR kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

Storage and Shelf life:

Part I of this kit should be stored at 2-8℃.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.

Reagent Foregene Protease Plus I has a unique formula. To ensure its better activity and stability, please store it at 4℃.

Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

Reagent 2×M-PCR Easy^TM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).