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Foregene Co., Ltd.
Foregene Co., Ltd.

Foregene Co., Ltd.

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Foregene Reverse Transcription Kits One Step RT-PCR Master Mix 100x20ul

Foregene Reverse Transcription Kits One Step RT-PCR Master Mix 100x20ul

  • Foregene Reverse Transcription Kits One Step RT-PCR Master Mix 100x20ul
  • Foregene Reverse Transcription Kits One Step RT-PCR Master Mix 100x20ul
  • Foregene Reverse Transcription Kits One Step RT-PCR Master Mix 100x20ul
Foregene Reverse Transcription Kits One Step RT-PCR Master Mix 100x20ul
Product Details:
Place of Origin: China
Brand Name: Foregene
Model Number: RT-02011/RT-02012
Payment & Shipping Terms:
Minimum Order Quantity: 100kits
Price: Negotiable
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 100000kits per month
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Detailed Product Description
Factory: Foregene Specifications: 100T (20μl System),500 T(20μl System)
Product Name: Quick And Accurate RT-PCR EasyTM I(One Step) Reverse Transcriptase Kit Other Name: One-step RT-PCR Master Mix
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Foregene Reverse Transcription Kits

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One step RT-PCR Master Mix 100x20ul

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Reverse Transcription Kits 100x20ul

 

Quick and accurate RT-PCR EasyTM I(One Step) Reverse Transcriptase Kit

 

 

Product Descprition

Foregene Reverse Transcription Kits One Step RT-PCR Master Mix 100x20ul 0

 

Foregene One-Step RT-PCR EasyTM series products realize the integrated reaction from RNA to double-stranded DNA, that is, reverse transcription and PCR amplification are completed in the same reaction centrifuge tube and the same reaction system, which simplifies the experimental steps, optimizes the experimental program, and improves the experiment efficiency.

RT-PCR EasyTM I(One Step) is a one-step RT-PCR kit specially developed by Foregene, which realizes continuous operation from RT to PCR in the same tube. The operation is simple and fast, which can effectively reduce the pollution during the experiment. 2×RT-PCR EasyTM Mix has strong tolerance, thermal stability, high efficiency and specificity for amplification.

 

Product components:

 

RT-PCR EasyTM I(One Step)
Kit contents RT-02011 RT-02012
100T (20μl system) 500T (20μl system)
2× RT-PCR EasyTM Mix 1ml 1.7ml×3
RNase-Free ddH2O 1.7ml 1.7ml×3
Instruction Manual 1 piece 1piece


Features&advantages:

  •  The one-step kit enables reverse transcription and PCR to be carried out in the same tube. It only needs to add template RNA, specific PCR primers and RNase-Free ddH2O.
  • Real-time quantitative analysis of RNA can be carried out quickly and accurately.
  • The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
  • The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.

 

Kit component information

2× RT-PCR EasyTM Mix:Foregene Reverse Transcriptase, RNase Inhibitor, Foregene HotStar Taq DNA Polymerase,dNTPs, Mg2+,reaction buffer, optimizer and stabilizer, etc.

 

Product quality control:

 

In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of RT-PCR EasyTM I(One Step) kits is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

 

 

Form 1:RT-PCR system preparation

 

RT-PCR system additions Amount Final Concentration
2× RT-PCR EasyTM Mix 10μl
Forward Primer (10μM) 0.5μl 0.2-0.25μM 1*
Reverse Primer (10μM) 0.5μl 0.2-0.25μM 1*
Template(RNA) Xμl 0.1pg-1μg
RNase-FreeddH2O (9-X)μl  
Total Volume 20μl  

 

1*: Usually the final concentration of primer is 0.2-0.25μM to get better results. When the reaction performance is poor, the primer concentration can be adjusted within the range of 0.1-0.5μM.

Note: Forward Primer and Reverse Primer are the specific primers for the target gene; 50μl system, please refer to the 20μl system to adjust the reagent dosage proportionally.

 

Storage and Shelf life:

 

The kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.

 

 

Contact Details
Foregene Co., Ltd.

Contact Person: Foregene

Tel: +8613880676215

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Foregene Co., Ltd.
Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
Tel:86-028-86050301
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