Foregene Reverse Transcription Kits One Step RT-PCR Master Mix 100x20ul

Name: Foregene Reverse Transcription Kits One Step RT-PCR Master Mix 100x20ul
Brand: Foregene
SKU: RT-02011/RT-02012
Rating: 5 (142 reviews)

Product Details:

Place of Origin:	China
Brand Name:	Foregene
Model Number:	RT-02011/RT-02012

Payment & Shipping Terms:

Minimum Order Quantity:	100kits
Price:	Negotiable
Packaging Details:	craft paper bag
Delivery Time:	5-8 working days
Payment Terms:	L/C, D/A, D/P, T/T
Supply Ability:	100000kits per month

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Detailed Product Description

Factory:

Foregene

Specifications:

100T (20μl System),500 T(20μl System)

Product Name:

Quick And Accurate RT-PCR EasyTM I(One Step) Reverse Transcriptase Kit

Other Name:

One-step RT-PCR Master Mix

Highlight:

Foregene Reverse Transcription Kits

One step RT-PCR Master Mix 100x20ul

Reverse Transcription Kits 100x20ul

Quick and accurate RT-PCR Easy^TM I(One Step) Reverse Transcriptase Kit

Product Descprition

Foregene Reverse Transcription Kits One Step RT-PCR Master Mix 100x20ul 0

Foregene One-Step RT-PCR Easy^TM series products realize the integrated reaction from RNA to double-stranded DNA, that is, reverse transcription and PCR amplification are completed in the same reaction centrifuge tube and the same reaction system, which simplifies the experimental steps, optimizes the experimental program, and improves the experiment efficiency.

RT-PCR Easy^TM I(One Step) is a one-step RT-PCR kit specially developed by Foregene, which realizes continuous operation from RT to PCR in the same tube. The operation is simple and fast, which can effectively reduce the pollution during the experiment. 2×RT-PCR EasyTM Mix has strong tolerance, thermal stability, high efficiency and specificity for amplification.

Product components:

RT-PCR Easy^TM I(One Step)
Kit contents	RT-02011	RT-02012
Kit contents	100T (20μl system)	500T (20μl system)
2× RT-PCR Easy^TMMix	1ml	1.7ml×3
RNase-Free ddH₂O	1.7ml	1.7ml×3
Instruction Manual	1 piece	1piece

Features&advantages:

The one-step kit enables reverse transcription and PCR to be carried out in the same tube. It only needs to add template RNA, specific PCR primers and RNase-Free ddH₂O.
Real-time quantitative analysis of RNA can be carried out quickly and accurately.
The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.

Kit component information

2× RT-PCR Easy^TMMix:Foregene Reverse Transcriptase, RNase Inhibitor, Foregene HotStar Taq DNA Polymerase,dNTPs, Mg²⁺,reaction buffer, optimizer and stabilizer, etc.

Product quality control:

In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of RT-PCR Easy^TM I(One Step) kits is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

Form 1:RT-PCR system preparation

RT-PCR system additions	Amount	Final Concentration
2× RT-PCR Easy^TM Mix	10μl	1×
Forward Primer (10μM)	0.5μl	0.2-0.25μM ¹^*
Reverse Primer (10μM)	0.5μl	0.2-0.25μM ¹^*
Template(RNA)	Xμl	0.1pg-1μg
RNase-FreeddH₂O	(9-X)μl
Total Volume	20μl

1*: Usually the final concentration of primer is 0.2-0.25μM to get better results. When the reaction performance is poor, the primer concentration can be adjusted within the range of 0.1-0.5μM.

Note: Forward Primer and Reverse Primer are the specific primers for the target gene; 50μl system, please refer to the 20μl system to adjust the reagent dosage proportionally.

Storage and Shelf life:

The kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.

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