Purity Mouse Tail Direct PCR Kit High DNA Extraction Yield PCR Kit without DNA Purification spin column purification

High DNA extraction yield and purity Mouse Tail Direct PCR Kit without prior DNA purification

Product Descprition

Due to the structure and properties of the mouse tail itself, the extraction of mouse tail genomic DNA has always troubled researchers: one is that the processing time of the mouse tail is long and the genomic DNA is easily degraded, the other is difficult to handle and the purity of the extracted genomic DNA is low.

High DNA extraction yield and purity Mouse Tail Direct PCR Kit without prior DNA purification uses a DNA-Only Column that can specifically bind DNA, a brand-new Foregene Protease and a unique buffer system, which can digest mouse tail samples within 45 minutes, thereby minimizing the degradation of genomic DNA and shortening sample processing time. The kit can extract 10-20μg of high-quality, high-purity genomic DNA from 0.5-1cm juvenile or adult mouse tails within 60-90min.

The DNA-only silica gel matrix material used in the spin column is a unique new material of the company, which can efficiently and specifically adsorb DNA. The maximum adsorption capacity for DNA is 80μg. The unique buffer and elution system can maximize the removal of RNA, Impurity proteins, ions and other organic compounds in the cell. The extracted genomic DNA fragment is large, high in purity, stable and reliable in quality, and the size of the DNA fragment is stable at about 23kb.

The kit can also be used to extract other mouse tissues outside the mouse tail, including mouse ears, mouse muscle, mouse liver and other tissues, so that one kit can be used for multiple purposes and meet the needs of users for different experiments.

Product INFO:

Kit components:

◆ Buffer TL1: Provide mouse tail enzymatic hydrolysis environment.

◆ Foregene Protease Plus: Enzymatically digest tissue samples in the environment of Buffer TL1.

◆ Buffer TL2: Inactivate Foregene Protease Plus and provide a DNA loading environment.

◆ Buffer PW: Remove impurities such as protein and RNA in DNA.

◆ Buffer WB: Remove residual salt ions in DNA.

◆ Buffer EB: Elute the DNA on the purification column membrane.

◆ DNA-only Column: Specifically adsorb genomic DNA in the lysate.

Genomic DNA extraction yield and purity:

The yield of mouse tail genomic DNA is related to sample size, storage conditions, degree of enzymolysis and operation. Mouse tail DNA Mini kit provides an effective and rapid method to obtain mouse tail genomic DNA. The obtained genomic DNA has high purity and low degradation, and OD₂₆₀/₂₈₀=1.7-1.9.

Genomic DNA fragment size:

Mouse Tail DNA Mini Kit uses a silica membrane column to isolate and purify mouse tail genomic DNA. The purified genomic DNA fragments size are all around 23 kb. As shown in the figure:

Notes: (Please read the notes carefully before using the kit):

◆ The sample should avoid repeated freezing and thawing, otherwise the extracted DNA fragments will be smaller and the extraction volume will also decrease.

◆ Before use, carefully check whether there is any precipitation in Buffer TL1, Buffer TL2 and Buffer PW. If there is precipitation, please dissolve it at 37°C and mix well before use.

◆ Before using the kit, be sure to check whether Buffer WB is added with absolute ethanol according to the instructions. Add 60ml absolute ethanol (DE-05211), 120ml absolute ethanol (DE-05212), and 300ml absolute ethanol (DE-053213) to Buffer WB before use.

◆ Elution volume: Buffer EB should not be less than 100μl, otherwise it will affect the DNA yield.

◆ Remember not to add RNase to any buffer.

◆ All centrifugation steps are centrifugation at room temperature (15-25℃) in a benchtop centrifuge.

◆ All experimental steps are carried out at room temperature (15-25℃).

Work Flow: